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HIV

1. For 10 HIV-1 proteins (Tat, Rev, and the products of the gag, pol and env genes), list the following. Your answer may take the form of a chart, if you find that convenient.

• location in viral particle, if applicable (you may attach and refer to a drawing)
• HIV-1 gene that encodes each (Tat and Rev have their own genes called tat and rev)
• form of mRNA that encodes each (fully spliced, partially spliced, unspliced)
• known functions in the HIV-1 life cycle
• host cell pathways required for expression

2. Diagram and explain the mechanism of HIV-1 reverse transcription. Include the 5' and 3' ends and the name of each strand (+ or - strand DNA, genomic RNA). Clearly label the sites of initiation of reverse transcription, the occasions and mechanisms of each "jump," and any instances of strand displacement. Include the role of the cPPT and the CTS.

3. Diagram and explain the mechanism of HIV-1 integration into the host cell genome. Identify the two known enzymatic activities of integrase protein and their role in integration.

4. Explain what is known about HIV-1 viral assembly in infected host cells, including how each component of the viral particle is trafficked to the membrane.

5. In terms that a lay person could understand, provide a detailed explanation of the role of the viral protease in maturation of HIV-1 virions.

Extra credit opportunity: This assignment is optional, for study purposes only. If you like, however, you may submit your answer to one of the above questions for consideration for extra credit. Choose the answer that you think is the best--I'm looking for exemplary answers. Each question is worth up to two points except number 2, which is worth up to four points. Your answer must be submitted by 5 pm on Wednesday 12/13 to be considered. The best answer to each question will be posted online as a study key.

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