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1. In broad outlines, describe the assay that is being used in figure 1. What is being measured? What is this measurement designed to reflect? How and why does this work?

2. Interpret the first line of figure 1B--the data for construct SE3.4K. What does each of the four horizontal bars represent? What do these data suggest?

3. Now compare construct SE3.4K to construct SE385. What does this comparison tell you? What about the comparison of SE385 and SE180?

4. How do the authors interpret the data for constructs SE385(deltaE) and SE83+E?

5. (2 pts) Your textbook discusses gel mobility shift assays (such as the ones presented in figure 3, figure 5, and figure 6C) on pages 346 -347. Consult this information, and then describe the data shown in figure 3B of the paper. What does this experiment show? Why is this significant?

6. (2 pts) What does figure 6B show? What do these data suggest about the mechanism of action of the dpn protein?

7. (2 pts) What is the underlying biological question addressed in this paper? Given that the sis-b gene is on the X chromosome of Drosophila, what do the authors' data suggest about this underlying question?

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