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HIV-1 Nuclear Import
and Integration
Wednesday, Dec 6,
2000
Announcements:
- Homework #11 is graded, and the key is online.
Please review this carefully, and let me know if you'd like to
have a special review session on this paper (Shibahara et
al).
- Homework #13 is online, and is
due at the beginning of class next Monday, 12/11/00
- Homework #14 (our HIV "chapter" homework) will be for study
purposes only
- Your final paper assignment for Bio312 is online.
A draft of this paper is due next week in lab, on 12/13/00.
- Because of conflicts for some students, I am offering you the
option of taking the Bio312 final exam early, at a time of your
choosing between 11 am and 6 pm on Monday 12/18. If you do not
make arrangments with me by Wed 12/13, I will assume that you will
take the exam as scheduled, on Tues 12/19 at 8:30 am.
Learning objectives for Wed 12/6/00
Students should be able to…
- Describe the current understanding of nuclear import of HIV-1.
Include the proposed roles of matrix, Vpr, and integrase, and the
role of the central DNA flap. Identify the host cell mechanisms
that would be required for this mechanism.
- Describe (and draw) the mechanism of integration of the HIV-1
provirus into the host cell genome. Include the role of both the
exonuclease and the strand transfer activities of integrase.
Supplemental figures for today
mechanism of HIV-1
integration (from Grandgenett and Mumm, "Unraveling Retrovirus
Integration", Cell 60 (1990), pp 3-4)
expression of the HIV-1
genome (from the AIDS Knowledge Base, chapter 3 section 2,
available online)
NOTE: The AIDS Knowledge Base is a very useful reference
and I highly recommend it. It is posted through the HIVInSite web
site at UCSF, which I've had periodic trouble accessing. If you
can get to it, though, it will be worth your while.
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Created by:
bkbaxter@lclark.edu
Updated: 6 Dec 00
