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Hoshijima et al: "Transcriptional Regulation of the Sex-lethal gene"

Announcements:

• Watch your email (and the web site)--I will soon be posting…

  • Keys to homework #8 (chapter 11) and homework #9 (Hoshijima et al)
  • Scans of your gels from this week's lab

• Monday: exam review--bring your questions (you can also email me over the weekend if you like)
• Monday 7 - 9pm: Midterm II

Learning objectives for Fri 11/10/00

Students should be able to…

1. (left over from Wed) Explain the role of sex-lethal protein in Drosophila sex determination. Include a description of its role in the expression of transformer and double-sex, and the effect of this on development
2. Explain the experimental design (in broad strokes, at least) and interpret any of the data presented in Hoshijima et al Figure 1
3. As above, for figure 3A. What is the radiolabeled probe in this experiment? The added protein(s)? The non-radioactive competitor DNA?
4. As above, for figure 4. What do these data show, and why is this significant? How did these data lead to the experiment in figure 6?
5. As above, for figure 6. Explain how these data show that deadpan is a DNA-binding protein, and why this is significant.

Outline:

• (review from Wed) Drosophila sex determination--the role of sex-lethal protein (as well as transformer and double-sex)
• Hoshijima et al--we'll take the figures roughly in order, paying particular attention to figures 1, 3, 4, and 6

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