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Mechanisms of Recombination

Announcements:

• Your lab stuff (except for the report that is due today) is now graded. A key to the week 9 report is online. Keys for the other weeks didn't seem necessary. As always, I am happy to discuss any of the reports with you.
• I've prepared a grade summary for each of you for Bio312. Remaining grades for lab include
  • another notebook check (individual, 12 points)
  • two more weekly lab reports, including the one due today (group, 10 points each)
  • an end-of-term report (group, 50 points), and
  • a final exam (individual, 75 points).
• More details about the final 312 report will be forthcoming after Thanksgiving. To prepare for the 312 final (which will be Tuesday, 12/19 at 8:30 am) your best bet is to review the lab reports from each week. Be sure that you understand (and can explain) all of the concepts covered in the reports, and you'll be OK on the final exam.

Learning objectives for Wed 11/22/00

Students should be able to…

1. Diagram and explain the mechanisms involved in: nonhomologous end-joining (fig 12-28), a simple Holliday model of recombination involving single-strand nicks (fig 12-29), and meiotic recombination via double-strand breaks (figure 12-31).
2. In reference to the above, use the following terms precisely and correctly: end resection, strand invasion, branch migration, exo vs. endonuclease cleavage, Holliday junction, resolution (of a Holliday junction), recombinant vs. nonrecombinant, heteroduplex.

Outline:

• We'll be building models of the following processes in class:
  • NHEJ (non-homologous end-joining),
  • a simple Holliday model of recombination, including branch migration and resolution, and
  • a model of meiotic recombination involving a double-strand break.

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