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Nuclear export and alternative splicing

Announcements:

• We'll begin lab today at 12:40 pm in Bio/Psych 137, with a 30-45 min strategy session/lab lecture
• Homework #9 is due on Friday in class--the reading is now available via on-line reserves, and good scans of the photos are available at the MoBio web site

Learning objectives for Wed 11/8/00

Students should be able to…

1. In broad terms, describe the role of the nuclear pore complex (NPC) in facilitating and regulating transport into and out of the nucleus
2. Using Ran as an example, explain how a slow (but activatable) GTPase activity is useful in regulating the activity of a G protein
3. Describe what is known about the export of processed messenger RNA's, including the direction of their transport through the NPC and the regulation of that transport
4. In broad terms (more details to come later) explain the role of the HIV Rev protein
5. Explain the role of sex-lethal protein in Drosophila sex determination. Include a description of its role in the expression of transformer and double-sex, and the effect of this on development

Outline:

• What we know about nuclear export so far: the role of the nuclear pore complex
• Nuclear export sequences and their recognition by proteins such as Exportin-1
• The role of Ran in nuclear trafficking
• What we know about mRNP export so far
• Spliceosome-associated mRNA's are not exported
• HIV rev overcomes this block
• Drosophila sex determination--the role of sex-lethal protein (as well as transformer and double-sex)

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